objective(s):some pathologic situations such as diabetes and metabolic syndrome are associated with alternation in nitric oxide level. incidence of these condition increases with aging. on the other hand, insulin secretion is modulated by nitric oxide, and nitric oxide synthase (nos) activity is also altered in diabetes. in this study, modification in the enzyme activity associated with aging a...

Comparison between Microplate Spectrometry and LC/MS Chromato-graphy for Facile Pilot Pharmacokinetics Biodistribution Studies of Doxorubicin-loaded Nanoparticle Drug CarriersPengxiao Cao Younsoo Bae

Abbreviations: PTMs=post-translational modifi cations; PPIs=protein-protein interactions; SDS-PAGE= sodium dodecyl sulfate-polyacrylamide gel electrophoresis; 2D-PAGE=two-dimensional polyacrylamide gel electrophoresis; MS=mass spectrometry; ESI=electrospray ionization; MALDI=Matrix Assisted Laser Desorption Ionization; ESI-MS=electrospray ionization mass spectrometry; MALDI-MS=MALDI mass spectr...

Changes in pigment content allow seagrasses to photo-acclimate to dynamic light environments, but lightand salinity-induced stress may reduce this ability. In this study, Thalassia testudinum seedlings were exposed to two light treatments (50 – 70% reduction and full sun) and three salinity treatments (20, 35, and 50) in a 44-day mesocosm experiment. Variation in leaf pigment content (chlorophy...

BACKGROUND In the European Union (EU), the use of diniconazole-M is no longer authorized. However, residues of diniconazole-M occur in various plant commodities. METHODOLOGY/PRINCIPAL FINDINGS A selective and simple analytical method for the trace level determination of diniconazole in soil, fruit, vegetables and water samples was developed based on immunoaffinity extraction followed by Enzym...

Within the framework of this study, a quantitative HPLC-MS/MS (high-performance liquid chromatography with mass spectrometric detection) method for determination vancomycin was developed. The in human plasma validated using chromato-mass method. accordance requirements EAEU bioanalytical methods. analytical range thetechnique 1–100 μg/mL plasma.

A highly sensitive and specific method for the detection of 06-methylguanine (O6-meG), O^-methylthymine (O4-meT), and CH-ethylthymine (O4-etT) has been established by combining prefractionation by high-performance liquid chromatography (III'IX '). '•'!' postlabeling, and immunoprecipitation by monoclonal antibodies (PREPI method). DNA was enzymatically hydrolyzed to 2'-deoxynucleoside-3'-mono...

Objective(s):Some pathologic situations such as diabetes and metabolic syndrome are associated with alternation in nitric oxide level. Incidence of these condition increases with aging. On the other hand, insulin secretion is modulated by nitric oxide, and nitric oxide synthase (NOS) activity is also altered in diabetes. In this study, modification in the enzyme activity associated with aging a...